Leucine-to-carbon empirical conversion factor experiments: does bacterial community structure have an influence?

Sample dilution and bacterial community composition influence empirical 1 leucine - to - carbon conversion factors in surface waters

Empirical leucine-to-carbon conversion factors for estimating heterotrophic bacterial production: seasonality and predictability in a temperate coastal ecosystem.

Leucine-to-carbon conversion factors (CFs) are needed for converting substrate incorporation into biomass production of heterotrophic bacteria. During 2006 we performed 20 dilution experiments for determining the spatiotemporal variability of empirical CFs in temperate Atlantic coastal waters. Values (0.49 to 1.92 kg C mol Leu(-1)) showed maxima in autumn to early winter and minima in summer. S...

Sample dilution and bacterial community composition influence empirical leucine-to-carbon conversion factors in surface waters of the world's oceans.

The transformation of leucine incorporation rates to prokaryotic carbon production rates requires the use of either theoretical or empirically determined conversion factors. Empirical leucine-to-carbon conversion factors (eCFs) vary widely across environments, and little is known about their potential controlling factors. We conducted 10 surface seawater manipulation experiments across the worl...

Bacterial production in the Delaware Bay estuary estimated from thymidine and leucine incorporation rates

The thymidine and leucine methods were examined and used to estimate bacterial production in the Delaware Estuary. During growth experiments that minimized grazing on bacteria, conversion factors for both thymidine and leucine were initially high and then rapidly decreased to values lower than commonly-used factors (2.0 X 10" cells mol-' for thymidine). The low thymidine conversion factors may ...

Large-scale variability in surface bacterial carbon demand and growth efficiency in the subtropical northeast Atlantic Ocean

We present surface estimates of bacterial respiration, bacterial heterotrophic production (BHP), and bacterial growth efficiency (BGE), and their relationship with nutrient availability, along a trophic gradient from coastal upwelling waters to the open-ocean waters of the eastern North Atlantic. Bacterial respiration generally ranged between 10 and 30 mg C L21 d21 and was relatively unaffected...